Testing culture media for pollen germination of elaeis. In vitro pollen germination effect of physical state of culture medium for in vitro pollen germination to select the best culture medium for the in vitro germination of pollen grains from passiflora, two liquid culture medium described for passiflora species bruckner et al. The high germination cultivars had welldeveloped pollen grains, whereas the low germination cultivars had a high percentage of aborted pollen grains fig. Proteins present at higher amounts in mature pollen were mainly those associated with tube. Take a few drops of this solution on a clear slide, dust continue reading study pollen germination on a slide. Pollen germination a challenging and educational experiment. Materials andmethods pollen used in these experiments was collected from freshly opened flowers of greenhouse lily, lilium longiflorulm thunb, cv. The aims was evaluation of pollen germination and pollen tube growth. To study the pollen germination on a slide portulacagrasschina rose. Prepare a nutrient solution by dissolving 10g sucrose and 10 mg boric acid in 100 ml water. Fresh seasonal flower slides, microscope, beaker, cavity slide, boric acid, sucrose.
September late had the highest pollen germination rate each year amongallcultivarstested from2012to2014,whereasga866,maya,andsherwood had the lowest. Standard methods for pollination research with apis mellifera. To study pollen germination on a slide using a microscope. Study of pollen germination on a slide working, procedure. Stained pollen grains were considered fertile, and unstained pollen grains were considered sterile. Hydrated pollen was then sprinkled on the surface using a.
At the time of liberation, it becomes 2 celled, with a. At the time of liberation, it becomes 2 celled, with a small generative cell and a vegetative cell. You can redo the experiment by clicking on the reset button. You will be able to prepare your practical file experiment very quickly and in a short time with the help of this lab practical handwritten notes pdf because all the experiments are related to the phone. Collect and study soil from at least two different sites and study them for texture, moisture content, ph and water holding capacity. Effects of various carbohydrates on the in vitro pollen germination of. Explore the technique and correct procedure required to perform this experiment. Pollen is a unique plant tissue that potentially can be used and manipulated to the advantage of the geneticist, breeder, physiologist, or germplasm curator.
Boric acid to certain extent also influences the in vitro pollen germination 56. Pdf effect of abscisic acid on pollen germination and tube. According to mean pollen germination, the highest value was obtained at ph. However, inhibitive effects of acidity degree on pollen germination and pollen tube elongation were in harmony with the first series of experiments. Drop only a few drops of the nutrient median 23 drops. As a whole, the pollen of the gramineae has proved very difficult to germinate. Pollen germination on solid surface hot agarcontaining medium was poured onto a microscope slide to form a layer with a thickness of about 0. Nov 20, 2017 pollen germination in vitro is a reliable method to test the pollen viability. Pdf on dec 7, 2016, gokul sivaraman published pollen germination methods find, read and cite all the research you need on. Microsporogenesis, microgametogenesis and in vitro pollen. Germination is one of the most important criteria of seed quality, because it affects crop establishment and therefore the yield and quality of the future harvest germination is a complex physiological mechanism. Pollen grains in plants are equivalent to sperm in animals and are therefore.
Pollen germination and tube growth are processes fundamental for reproduction of flowering plants. Holm department of ecological botany, university of umed, s901 87 umed, sweden. Pollen, germination, elongation, bauhinia blakeane. As com pared to the control, 50 m aba significantly experiment 1. In this experiment to study pollen germination on a slide the germinated pollen grains are viable. When you study pollen germination on a slide keep these things in mind. Pollen germination occurred over tbe first 10 min and pollen tube growtb continued for 45 min table 1. In vitro germination of wheat pollen on raffinose medium. Materials table sugar and salts for stock solutions pipette. Pollens was stored in desiccators and cultured in a medium including %15 sucrose, 100 ppm boric acid and %0. The growing ovule then transforms itself into a seed.
The only difference between the first and second series was that the acid rain started to inhib it pollen tube elongation at ph 3. It is concluded that selection among male gametes may occur after. Five to six flowers were used for each assay and at least two replicate experiments were done. In vitro pollen quantity, viability and germination tests in. On medium witb 075 m raffinose, pollen wbicb burst did so witbin 5 min of tbe cultures being set up fig. The four experiments described below probably best represent the findings of this study. Quantifying pollen deposited on a receptive stigma per visit or unit time 12 3. Optimization of conditions for germination of coldstored. In our experiments, the germination of pollen of e. In this experiment to study pollen germination on a slide, start the procedure by creating the nutrient solution. The slides were dehydrated by a gradually increasing ethyl alcohol series and, then they were passed through an ethyl alcoholxylene series and trans. Pollen grain germination on slide experiment youtube. Pollen germination an overview sciencedirect topics.
Assessment of pollen viability, germination, and tube growth. It also addresses many basic questions in sexual reproduction and particularly useful in wide hybridization. Among the stain tested, acid fuschin 1% was found to be effective fig. Study on pollen germination and pollen tube growth of five. Collect and study soil from at least two different sites and study them for texture, moisture content,ph and water holding. Vivavoce study of pollen germination as per pattern of cbse 201819 q. Flower, needles, safranine stain, glycerine, coverslips, microscope. In 1994 additional germination experiments were performed with pollen mixtures consisting of pollen from two different parental trees in known weight proportions. Leica application suite software was used to measure the length of areca pollen tube. Pollen quality is normally measured 1 by scoring seed set in plants fertilized with a particular pollen sample, 2 by cytochemical staining of the grains, or 3 by looking at germination of the pollen. Summarizes the recent research on pollen germination and introduces some basic studies on pollen tube growth that can be conducted in a secondary school laboratory. Students do the experiment better in the real lab having gone through the animation and simulation.
This experiment was done under field conditions in 1968 and there were three laterals per cross. Conclusion germination is one of the most important. We shall estimate the optimum concentration for maximum pollen grain germination by using an analysis of variance and polynomial. To calculate the pollen viability by histochemical test in the laboratory. Observation several pollen grains germinate and put forth pollen tubes. We supplied our students this video for them to selfstudy and prepare themselves before conducting the experiment about pollen germination. In an experiment conducted by luza and polito 1985, maximum walnut pollen germination was obtained on a culture medium solidified with 0. It is light weight, lacks thick walls, has a dormancy period, grows quickly under. All the dyes used in this experiment foreichhornia cras. In vitro germination and pollen tube growth of maize zea. Freshly plucked seasonal flowers, beaker, boric acid, sucrose, microscope and cavity slide. The factorial experiment was blocked in time with 3 replications one petri.
Pollens were dispersed on a medium containing 15% sucrose and. In the present study, in vitro pollen germination and pollen tube growth of all cultivars were severely reduced under both high and low temperature conditions. The first step involves the preparation of a nutrient solution. While experiments with the pollen of many plants have resulted not only in ready germination but in satisfactory methods of. Pollen grains show great variation in their morphology and structure. Study of pollen germination on a slide working, procedure and. Conclusion germination is one of the most important criteria.
Class xii cbse biology practical study pollen germination on. Whatever the interest and use, the viability of the pollen is. Ace, except where otherwise specified table iii, fig. To study the pollen grain germination on stigma through a permanent slide. In the first experiment, a sucrose gradient was used at 20 concentrations in 0. The artificial germination of plant pollens has met with varying suc cess. Request pdf pollen germination a challenging and educational experiment pollen is a unique plant part. We can stimulate pollen germination in vitro with the help of a nutrient medium. The pollen will pass by sperm tube for fertilizing the ovum. Discusses the use of a light microscope and refrigerator to study pollen. Many pollen germination medium ranging from simple sugars to complex one having vitamins, growth regulators, etc. Pollination density effects on pollen germination and pollen tube growth in betula pubescens ehrh. Lets see how pollen germinates in flowering plants, however, the ovules are contained within a hollow organ called the pistil, and the pollen is deposited on the pistils receptive surface, the stigma.
The pollen grain is uninucleate has one nucleus in the beginning. The third question will be answered from the second experiment. In vitro germination of pepper pollen in liquid medium. All the biology experiments of class xii are explained very well in this book pdf. Study pollen germination on a slide biology class 12. Olga pechanova, tibor pechan, in proteomics in food science, 2017. A total of 3000 pollen grains, both stained and unstained, were counted in 10 randomly chosen regions, and pollen viability was calculated. In nature, pollen germination takes place on the basis of a flowers stigma and the pollen tube elongate toward the ovule of the flower. Pollen germination on stigma through a permanent slide. Weaver and timm 1988 suggested that pollen is ore sensitive to high temperatures than femal. Influence of boric acid and sucrose on the germination and.
This is done by dissolving 10g of sucrose as well as 10mg of boric acid in 100ml of water. Pollen collected from 44 genotypes grown outdoors in large pots was subjected to in vitro temperature treatments that varied from 15 to 50 c at 5 c increments in. Therefore, pollen grain was collected from unopened flowers of iran apricot cultivars including. The pollen was observed at 60 x magnification with a stereo binocular microscope to evaluate germination. Pollen tube growth, and fruit development in tomato and cucumber. In vitro pollen viability and pollen germination in cherry. Since pollen of most species is haploid and each cell is independent, pollen has the potential to provide a microbiallike system for evaluation, assay, and selection. Tabulate your observations and calculate the percentage of pollen germination. Pollen germinationa challenging and educational experiment.
Abstract many aspects of angiosperm pollen germination and tube growth are discussed including mechanisms of dehydration and rehydration, in vitro germination, pollen coat compounds, the dynamic involvement of cytoskeletal elements actin, microtubules, calcium ion fluxes, extracellular matrix elements stylar arabinogalactan proteins, and control mechanisms of gene expression in dehydrating. To demonstrate the hanging drop method of pollen germination. Significant differences at each storage period and a differential response to storage were obtained at some loci for germination percentage, ruptured percentage, pollen tube length and fertilization ability. Pollen germination tests concentrated agar 1% in petri dish containing different concentrations of sucrose 0, 5, 10, 15 and 20 mgl were used during the first pollen germination experiment.
Pollen viability was determined on the basis of different staining methods, pollen germination potential on stigma or invitro germination of pollen grains rodriguezriano and dafni, 2000. The tube cell enlarges and comes out of the pollen grain through one of the germ pores to form a pollen tube. Click on the eyepiece of the compound microscope to view the pollen germination. Although the germination rate increases at boric acid concentrations of 0. The effect of temperature on in vitro pollen germination and pollen. For pollen germination, pollen grains were collected by immediately dehiscing anthers. In vitro arabidopsis pollen germination and characterization. Seed to be sure have other functions in modern agriculture. Pollen germination with fast plants pollen from fast plants can be stimulated to germinate in a sucrosesalt solution maintained as a hanging drop slide. Dependence on standing water for fertilization in seed plants has been reduced by pollen. The essential role of different factors in pollen germination.
Six flowers of each lateral were used, two for each dilution of pollen. Jan 01, 2010 during lily pollen maturation, amyloplast membranes start to disappear gradually, and starch granules are scattered in the cytoplasm, but redifferentiation of proplastids to amyloplasts is observed in the cytoplasm of lily pollen after 30min germination and is continuously present in the cytoplasm of elongating pollen tubes jiang et al. Forty eight slides from six flower buds and eight sucrose concentrations were prepared in the first experiment for microscopic examination of pollen germination rates. In nature, pollen grains germinate on the compatible stigmas of the carpel. How closely does pollen germination rate correlate with pollen grain viability rate. Increasing agar concentration in the medium resulted in a decline in pollen germination. Pollination density effects on pollen germination and. To study the pollen grains of some plants belonging to monocotyledons. It has been studied for many years and more and more studies are being conducted at a molecular level in order to more fully. Holm department of ecological botany, university of umed, s901 87 umed, sweden received 5 july 1993. After seeing the germination in your control pollen, pick the flowers which worked the best, and continue the rest of the experiment, but also do tradescantia since that will be used in. It represents both the fulfillment and the completion of the basic function of seed propagation. On the stigma, the germination of pollen grains begins by absorption of water and nutrients and the pollen grain produces a tiny pollen tube through the style to the ovary. In vitro pollen germination ofeichhornia crassipes mart.
Experiment 1 will attempt to answer the first two questions. Germinating and mature maize pollens were compared via gelbased proteomic tools zhu et al. Simple methods for in vitro pollen germination and pollen. Within 30 to 40 minutes, early pollen tubes can be observed under 40x magnification, growing from the pollen grains. Count the total number of pollen grains and the number of germinated pollen grains in 35 different microscope fields. Over 100 pollen grains were examined for every treat. Evaluating pollen identity, quantity and quality on stigmas 12 3. Delouche1 germination is the crucial and final event in the life of a seed. The science which deals with the study of the pollen grain is called palynology. Areca pollen germination rate % germinated areca pollen grain numbertotal areca pollen grain number. A standardization of pollen germination medium for c. To study pollen germination and growth of pollen tube. On the stigma, the germination of pollen grains begins by absorption of water and nutrients and the pollen grain produces a tiny pollen.
Do not use dirty slides for dusting pollens, make sure it dry and clean. Study pollen germination on a slide biology class 12 experiments. Pollen germination introduction germination of pollen occurs when a pollen grain forms a tube of pollen. Class xii cbse biology practical study pollen germination. The experiment was designed so that comparisons free from genetic background effects could be made between alleles at each locus.
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